By Archer K.J., Dumur C.I., Joel S.E.
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Extra info for Assessing quality of hybridized RNA in Affymetrix GeneChip experiments using mixed-effects models
3. DATA ANALYSIS AND QUESTIONS 1. Plot ÁP ¼ fðtÞ for the first stage at constant flow rate. Verify if the relationship is linear (cake filtration law at constant flow rate) and determine r from the slope. 2. Plot dV dt ¼ fðtÞ for the second stage at constant ÁP. Filtration II – Using a Plate-and-Frame Filter 33 3. Calculate Vt ¼ fðVÞ through numerical integration for the second stage. Verify if the relationship is linear (cake filtration law at constant ÁP) and determine r from the slope. 4. Confirm the validity of the cake filtration law.
They can be obtained from water removal rate (rate of mass increase of osmotic medium or mass decrease of raw material, kg h–1) divided by total membrane area (m2). 2. This data can be correlated to process conditions to explore the impact of process conditions. 3. Calculations Based on measured flux values, calculation of the overall mass transfer coefficient (U) is carried out by using the fundamental mass transfer equation: U¼ (5:1) Flux ÁÅ À ÁP where Flux is the flow rate (kg m–2 h–1), and ÁÅ is the osmotic pressure difference across the membrane (bars).
C. 2. Schematic illustration of the filterability apparatus. Thermometer (–10 to 1108C). Stop Clock (reads seconds to minutes). 100-mm diameter Funnel (for reverse flow filling). Leighton Buzzard sand. Kaolin. Alum. 3. Procedure 1. S. S. 55 mm. 2. Fill the apparatus with 40 mm depth of granular media of known porosity in the Perspex column (A). 3. Pour clear water up to the base of the inlet funnel. 4. Prepare a 100 mgÁL–1 suspension of kaolin in tap water. 5. Add 5 mgÁL–1 of alum to flocculate the suspension.
Assessing quality of hybridized RNA in Affymetrix GeneChip experiments using mixed-effects models by Archer K.J., Dumur C.I., Joel S.E.