By Donna Latham
Bridges and tunnels are lifelines. humans have tackled likely insurmountable stumbling blocks, together with sizeable canyons and mountain levels, to layout and build those impressive passageways. Bridges and Tunnels: examine Feats of Engineering invitations young children a long time nine and as much as discover the innovation and actual technological know-how at the back of constructions our international will depend on. trivialities and enjoyable proof illustrate engineering ingenuity and achievements. actions and initiatives motivate childrens to profit concerning the engineering approach and to embody trial and blunder.
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Extra info for Bridges and Tunnels: Investigate Feats of Engineering with 25 Projects (Build It Yourself Series)
Allow to stand for 20 min, dilute to 100 ml and measure solution A colorimetrically using the Spekker photo-electric absorptiometer with red (No. 8) filters and 1 cm glass cells against solution B as the blank. Construct a calibration chart of Spekker reading against concentration of cystine (mg/ml). (c) Cystine analysis of wool—Approx. 0 2 5 g of wool at atmospheric regain is dried by spreading out in an oven for 1 h at 110°C or in an evacuated desiccator over phosphorus pentoxide for 2 days. The dry weight, which should be between 0 1 8 and 0-22 g, is accurately determined.
Sodium chloride by making up sodium chloride (5-85 g) to 1 litre with water and diluting 1 0 0 ml of the resulting solution to 1 litre. Using this solution as solvent, determine the R values of the amino acids as in normal partition paper chromatography (page 31). Spray the paper with 1 per cent acetic acid solution prior to locating the spots with ninhydrin to neutralise the alkalinity of the paper and provide the correct pH conditions for colour development. Using a fresh sheet of DE81 paper, repeat the separation using as solvent n-butanol-acetic acid-water (60:15:25 by volume) which suppresses the ion-exchange characteristics of the paper and the separation is achieved by conventional partition chromatography.
EXPERIMENTAL P R O C E D U R E (a) Preparation of phosphotungstic acid reagent—Dissolve sodium tungstate (25 g) in water (50ml) and add phosphoric acid ( 5 0 m l ) slowly with cooling. Pass hydrogen sulphide through the solution at a moderate rate for 20min. ) After the hydrogen sulphide has been passing for 3-4 min, add more phosphoric acid (2-5 ml) without interrupting the gas flow. Filter the solution, if necessary re-filtering the first few millilitres of the filtrate. The filtrate should be clear and slightly green.
Bridges and Tunnels: Investigate Feats of Engineering with 25 Projects (Build It Yourself Series) by Donna Latham